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basic grammar problem

basic grammar problem

1)What is the differences between these two sentences? Which one is correct
- I have no issue.
- I doesn't have issue.

2)in/at? In this case, which one is more suitable to use?why?
- Please take the file in PC01.
- Please take the file at PC01.

3)because and as? What is the differences?Are both suitable use in all sentence?